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rps3-rps19

LSC · 91932-92694 · 763 bp

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Pi 0.284

MarkerSeek score 82.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 187
pi: 0.284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TGAACTCCTTTTGTACCCGC	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1705-1744 bp	1.0	90.7
rps3-rps19_p2	GGTGTCCATAAGGGTTTGCA	ATAAACTTCCGGGGACATGC	50.0	50.0	58.1	57.9	1181 bp	0.5	58.9
rps3-rps19_p3	GGTGTCCATAAGGGTTTGCA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1593 bp	0.5	58.9
rps3-rps19_p4	ACGTCCCGCAATTTGTACTT	ATAAACTTCCGGGGACATGC	45.0	50.0	58.1	57.9	1312 bp	0.5	58.7
rps3-rps19_p5	TGAACTCCTTTTGTACCCGC	ATAAACTTCCGGGGACATGC	50.0	50.0	58.1	57.9	1293 bp	0.5	58.7