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psbE-petL

LSC · 72069-73355 · 1287 bp

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Pi 0.0641

MarkerSeek score 79.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1287
variable_sites: 71
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.0	56.3	1296-1444 bp	1.0	74.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1285-1433 bp	1.0	74.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1208-1356 bp	1.0	73.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1203-1351 bp	1.0	73.2
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1287-1435 bp	1.0	72.6