Hotspot

accD-psaI

LSC · 66084-66640 · 557 bp

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Pi 0.101

MarkerSeek score 80.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 56
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCATGCTTTCTTTCCTT	AGAGGGTAAGTTGCTGAGAGT	45.0	47.6	56.3	58.1	618-803 bp	1.0	64.3
accD-psaI_p2	GCTCCATGCTTTCTTTCCTT	GGGTAAGTTGCTGAGAGTTGT	45.0	47.6	56.3	57.9	615-800 bp	1.0	64.1
accD-psaI_p3	GCTCCATGCTTTCTTTCCTTT	AGAGGGTAAGTTGCTGAGAGT	42.9	47.6	57.1	58.1	618-803 bp	1.0	62.3
accD-psaI_p4	GCTCCATGCTTTCTTTCCTTT	GGGTAAGTTGCTGAGAGTTGT	42.9	47.6	57.1	57.9	615-800 bp	1.0	62.2
accD-psaI_p5	GCTCCATGCTTTCTTTCCTT	AGGGTAAGTTGCTGAGAGTTG	45.0	47.6	56.3	57.6	616-801 bp	1.0	61.9