Hotspot

ndhC-trnV-UAC

LSC · 57203-58128 · 926 bp

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Pi 0.0823
MarkerSeek score 84.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
926
variable_sites
70
pi
0.0823
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTCGA AGAAGGTCTACGGTTCGAGT 37.5 50.0 58.0 57.8 1031-1168 bp 1.0 55.4
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTCG AGAAGGTCTACGGTTCGAGT 39.1 50.0 56.7 57.8 1031-1168 bp 1.0 52.9
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTCGA GAAGGTCTACGGTTCGAGTC 37.5 55.0 58.0 57.5 1030-1167 bp 1.0 52.6
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTCGA GTTCGAGTCCGTATAGCCCT 37.5 55.0 58.0 58.7 1019-1156 bp 1.0 51.6
ndhC-trnV-UAC_p5 GCCAAAATAGGAATAAGACTCGA AGAAGGTCTACGGTTCGAGT 39.1 50.0 56.4 57.8 1030-1167 bp 1.0 50.9