Hotspot

trnT-UGU-trnL-UAA

LSC · 52183-53649 · 1467 bp

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Pi 0.0785

MarkerSeek score 78.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1467
variable_sites: 93
pi: 0.0785
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1336-1549 bp	1.0	87.2
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1344-1557 bp	1.0	86.6
trnT-UGU-trnL-UAA_p3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.4	58.0	1308-1521 bp	1.0	86.4
trnT-UGU-trnL-UAA_p4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.4	58.1	1316-1529 bp	1.0	85.8
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1350-1563 bp	1.0	85.3