Hotspot

psbZ-trnG-GCC

LSC · 41650-42255 · 606 bp

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Pi 0.0534
MarkerSeek score 79.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
606
variable_sites
31
pi
0.0534
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG TACCATTCGACTATATCCGC 55.0 45.0 58.0 54.3 733-912 bp 1.0 55.6
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG TTACCATTCGACTATATCCGC 55.0 42.9 58.0 55.1 734-913 bp 1.0 54.3
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG TACCATTCGACTATATCCGC 50.0 45.0 57.8 54.3 736-915 bp 1.0 54.1
psbZ-trnG-GCC_p4 TCCTGATGGTTGGTCGAGTA TACCATTCGACTATATCCGC 50.0 45.0 57.8 54.3 729-908 bp 1.0 54.0
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC TACCATTCGACTATATCCGC 55.0 45.0 58.3 54.3 734-913 bp 1.0 53.7