Hotspot

trnS-UGA-psbZ

LSC · 41127-41460 · 334 bp

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Pi 0.0545

MarkerSeek score 81.60

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 334
variable_sites: 17
pi: 0.0545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.0	58.0	437-457 bp	1.0	86.7
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	444-464 bp	1.0	86.3
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	445-465 bp	1.0	86.3
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	452-472 bp	1.0	86.2
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	434-454 bp	1.0	85.2