Hotspot

rpoB-trnC-GCA

LSC · 30094-31314 · 1221 bp

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Pi 0.0432
MarkerSeek score 81.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1221
variable_sites
48
pi
0.0432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ACATTCCCGCATTTACACCC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.5 58.1 1255-1311 bp 1.0 82.1
rpoB-trnC-GCA_p2 ACATTCCCGCATTTACACCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.5 58.4 1237-1293 bp 1.0 80.0
rpoB-trnC-GCA_p3 GCATTTACACCCCCAAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 58.8 58.1 1247-1303 bp 1.0 79.9
rpoB-trnC-GCA_p4 GACATTCCCGCATTTACACC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.2 58.1 1256-1312 bp 1.0 79.6
rpoB-trnC-GCA_p5 TGTATCTGATTCAACCCGGG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.0 58.1 1283-1339 bp 1.0 78.3