Hotspot

rps16-trnQ-UUG

LSC · 7139-7791 · 653 bp

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Pi 0.0881

MarkerSeek score 79.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 51
pi: 0.0881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	733-782 bp	1.0	88.9
rps16-trnQ-UUG_p2	TGATTCCCCCAGATCCACTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1073-1126 bp	1.0	87.7
rps16-trnQ-UUG_p3	GTTCCACGATCCGTTAGCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1208-1260 bp	1.0	87.4
rps16-trnQ-UUG_p4	CACTCGTATTCGCAACCTCA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1412 bp	0.5	59.3
rps16-trnQ-UUG_p5	TCCATCATCAAACCCTCCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	870 bp	0.5	59.3