Hotspot

trnK-UUU-rps16

LSC · 4416-5994 · 1579 bp

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Pi 0.136

MarkerSeek score 82.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1579
variable_sites: 168
pi: 0.136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	GGCGGGTGTTTTTATGGAAC	50.0	50.0	58.6	57.6	1648-1739 bp	1.0	84.5
trnK-UUU-rps16_p2	GAGTCGCACTTAAAAGCCGA	GGCGGGTGTTTTTATGGAAC	50.0	50.0	58.6	57.6	1649-1740 bp	1.0	84.5
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	GGCGGGTGTTTTTATGGAAC	45.0	50.0	57.3	57.6	1646-1737 bp	1.0	83.3
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGCGGGTGTTTTTATGGAAC	50.0	50.0	57.2	57.6	1645-1736 bp	1.0	82.8
trnK-UUU-rps16_p5	GTCGCACTTAAAAGCCGAGT	GGCGGGTGTTTTTATGGAAC	50.0	50.0	58.9	57.6	1647-1738 bp	1.0	82.2