Hotspot

ycf1

IRb · 111506-113314 · 1809 bp

Back to Zanthoxylum

Pi 0.00889

MarkerSeek score 48.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1809
variable_sites: 80
pi: 0.00889
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	AATCCAACCCTTTCCGTCAG	55.0	50.0	58.0	57.8	1752-2456 bp	1.0	86.7
ycf1_p2	CCGGAACTTCTTCGTAGTGG	AATCCAACCCTTTCCGTCAG	55.0	50.0	58.0	57.8	1751-2455 bp	1.0	86.7
ycf1_p3	CCCGGAACTTCTTCGTAGTG	ACTAACGCATTGGTTCGACA	55.0	45.0	58.0	57.8	1513-2217 bp	0.935484	83.5
ycf1_p4	CCGGAACTTCTTCGTAGTGG	ACTAACGCATTGGTTCGACA	55.0	45.0	58.0	57.8	1512-2216 bp	0.935484	83.5
ycf1_p5	TCCCGGAACTTCTTCGTAGT	ACTAACGCATTGGTTCGACA	50.0	45.0	58.1	57.8	1514-2218 bp	0.935484	82.9