Hotspot

rps3

LSC · 84641-85300 · 660 bp

Back to Zanthoxylum

Pi 0.00792

MarkerSeek score 44.50

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 660
variable_sites: 37
pi: 0.00792
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TACTACAGGACGGTCCGTTT	TCCCGGGCATCTACCATTAT	50.0	50.0	58.1	58.0	1406-1593 bp	0.967742	87.0
rps3_p2	ATACTACAGGACGGTCCGTT	TCCCGGGCATCTACCATTAT	50.0	50.0	57.9	58.0	1407-1594 bp	0.967742	86.7
rps3_p3	TACTACAGGACGGTCCGTTT	GGGTTTCAACGAAGGGAGTT	50.0	50.0	58.1	58.0	1134-1162 bp	0.935484	82.6
rps3_p4	ATACTACAGGACGGTCCGTT	GGGTTTCAACGAAGGGAGTT	50.0	50.0	57.9	58.0	1135-1163 bp	0.935484	82.2
rps3_p5	CTACAGGACGGTCCGTTTTT	GGGTTTCAACGAAGGGAGTT	50.0	50.0	57.8	58.0	1132-1160 bp	0.935484	82.1