Hotspot

ndhE-ndhG

SSC · 121113-121363 · 251 bp

Back to Zanthoxylum

Pi 0.0199

MarkerSeek score 69.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 25
pi: 0.0199
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGTTCAAGACACATCAGGGC	TTGATAGGTGCAATTGCCGT	50.0	45.0	57.8	58.2	370-397 bp	1.0	86.7
ndhE-ndhG_p2	CGAACCATATTTCGGCTCGT	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.4	58.2	349-376 bp	1.0	85.1
ndhE-ndhG_p3	CAGGGCCCGAACCATATTTC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.7	58.2	356-383 bp	1.0	83.0
ndhE-ndhG_p4	GTTCAAGACACATCAGGGCC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.8	58.2	369-396 bp	1.0	81.6
ndhE-ndhG_p5	ATCAGGGCCCGAACCATATT	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.9	58.2	358-385 bp	1.0	81.6