Hotspot

ndhF-rpl32

SSC · 115419-116393 · 975 bp

Back to Zanthoxylum

Pi 0.0233
MarkerSeek score 70.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
975
variable_sites
117
pi
0.0233
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACTGGGCCGGCAAATAATAG GAACTGTTGTAGCGGCTGTA 50.0 50.0 58.0 57.9 2282-2385 bp 0.903226 86.7
ndhF-rpl32_p2 TGTTGCGGTCGGAAAAAGTA TTTAGGGTGTATGGTGGGGT 45.0 50.0 58.0 57.9 1455-1563 bp 0.870968 85.9
ndhF-rpl32_p3 ACTGGGCCGGCAAATAATAG TTTGAGTCAGGCGGATTCAG 50.0 50.0 58.0 57.9 1807-1908 bp 0.903226 85.7
ndhF-rpl32_p4 ACTGGGCCGGCAAATAATAG TTTAGGGTGTATGGTGGGGT 50.0 50.0 58.0 57.9 2069-2177 bp 0.870968 85.2
ndhF-rpl32_p5 ACTGGGCCGGCAAATAATAG CTGTTGTAGCGGCTGTAACT 50.0 50.0 58.0 57.9 2279-2382 bp 0.870968 85.2