Hotspot

accD-psaI

LSC · 60497-61195 · 699 bp

Back to Zanthoxylum

Pi 0.0162

MarkerSeek score 73.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 62
pi: 0.0162
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGTGTTCTGAGCGAGT	AAAGAAGCCATTGCCATTGC	45.0	45.0	57.4	57.9	775-891 bp	1.0	84.5
accD-psaI_p2	AAAGGTGTTCTGAGCGAGTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	57.4	57.9	774-890 bp	1.0	84.5
accD-psaI_p3	AAAGGTGTTCTGAGCGAGTT	TTGCCATTGCCGGAAATACT	45.0	45.0	57.4	58.2	764-880 bp	1.0	84.2
accD-psaI_p4	AAAAGGTGTTCTGAGCGAGT	TTGCCATTGCCGGAAATACT	45.0	45.0	57.4	58.2	765-881 bp	1.0	84.1
accD-psaI_p5	AAAAGGTGTTCTGAGCGAGTT	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	775-891 bp	1.0	80.8