Hotspot

ndhC-trnV-UAC

LSC · 52787-53203 · 417 bp

Back to Zanthoxylum

Pi 0.0145

MarkerSeek score 71.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 43
pi: 0.0145
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTGGCCCTTTGTTAATCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	566-613 bp	1.0	87.5
ndhC-trnV-UAC_p2	TAATCGGGGCTAAAACTGCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	553-600 bp	1.0	85.8
ndhC-trnV-UAC_p3	TCTGGCCCTTTGTTAATCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	565-612 bp	1.0	84.7
ndhC-trnV-UAC_p4	TCTGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	554-601 bp	1.0	83.7
ndhC-trnV-UAC_p5	TAATCGGGGCTAAAACTGCG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.3	57.5	552-599 bp	1.0	83.1