Hotspot

psbZ-trnG-GCC

LSC · 38099-38597 · 499 bp

Back to Zanthoxylum

Pi 0.034

MarkerSeek score 74.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 68
pi: 0.034
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	638-827 bp	1.0	87.7
psbZ-trnG-GCC_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	648-837 bp	1.0	86.7
psbZ-trnG-GCC_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	649-838 bp	1.0	86.3
psbZ-trnG-GCC_p4	GGATGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	637-826 bp	1.0	84.5
psbZ-trnG-GCC_p5	CGTTGTATTTGCTTCTCCGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	655-844 bp	1.0	83.9