Hotspot

trnD-GUC-trnY-GUA

LSC · 31923-32412 · 490 bp

Back to Zanthoxylum

Pi 0.0164
MarkerSeek score 70.30
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
490
variable_sites
47
pi
0.0164
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 325-570 bp 1.0 85.1
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 324-569 bp 1.0 85.1
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 346-591 bp 1.0 84.0
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 345-590 bp 1.0 83.8
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.5 58.4 379-624 bp 1.0 83.1