Hotspot

trnC-GCA-petN

LSC · 29184-29902 · 719 bp

Back to Zanthoxylum

Pi 0.0136

MarkerSeek score 69.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 60
pi: 0.0136
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.4	58.1	806-848 bp	1.0	86.2
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CCATACTACGAGCGAAAGGG	50.0	55.0	58.4	57.9	813-855 bp	1.0	85.9
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CCCATACTACGAGCGAAAGG	50.0	55.0	58.4	57.9	814-856 bp	1.0	85.9
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CCCCATACTACGAGCGAAAG	50.0	55.0	58.4	57.9	815-857 bp	1.0	85.9
trnC-GCA-petN_p5	ATTTTGGATCGTTTTGGCGG	ACGAGCGAAAGGGAAAATGT	45.0	45.0	57.6	58.1	845-887 bp	1.0	85.8