Hotspot

trnS-GCU-trnG-UCC

LSC · 8711-9688 · 978 bp

Back to Zanthoxylum

Pi 0.0211

MarkerSeek score 75.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 129
pi: 0.0211
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	889-1080 bp	1.0	64.7
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	886-1077 bp	1.0	63.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.6	947-1138 bp	1.0	63.5
trnS-GCU-trnG-UCC_p4	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	888-1079 bp	1.0	62.6
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.3	57.6	886-1077 bp	1.0	62.6