Hotspot

rps16-trnQ-UUG

LSC · 6101-7374 · 1274 bp

Back to Zanthoxylum

Pi 0.0177
MarkerSeek score 69.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1274
variable_sites
126
pi
0.0177
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 355-1365 bp 1.0 91.2
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 353-1363 bp 1.0 91.2
rps16-trnQ-UUG_p3 CAGCGGATCATGTCCTTCAA GAAATTGAAATGGGGCGTCG 50.0 50.0 58.0 58.1 413-1423 bp 1.0 91.0
rps16-trnQ-UUG_p4 GCGGATCATGTCCTTCAAGT GAAATTGAAATGGGGCGTCG 50.0 50.0 58.0 58.1 411-1421 bp 1.0 91.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 337-1347 bp 1.0 90.3