Hotspot

rps3

LSC · 88623-89309 · 687 bp

Back to Zantedeschia

Pi 0
MarkerSeek score 37.60
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
687
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 TCACTGGATAGAATGGTAAGACA AGCGACCCACCTGTCATATA 39.1 50.0 56.5 57.9 842 bp 1.0 43.1
rps3_p2 TCACTGGATAGAATGGTAAGACAA AGCGACCCACCTGTCATATA 37.5 50.0 57.1 57.9 842 bp 1.0 40.4
rps3_p3 CACTGGATAGAATGGTAAGACA AGCGACCCACCTGTCATATA 40.9 50.0 55.0 57.9 841 bp 1.0 40.0
rps3_p4 TGGTAAGACAAATAAAGGTCCA AGCGACCCACCTGTCATATA 36.4 50.0 55.0 57.9 829 bp 1.0 40.0
rps3_p5 ATCACTGGATAGAATGGTAAGACA AGCGACCCACCTGTCATATA 37.5 50.0 56.9 57.9 843 bp 1.0 40.0