Hotspot

rpl22

LSC · 89356-89739 · 384 bp

Back to Zantedeschia

Pi 0.0026

MarkerSeek score 55.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 384
variable_sites: 1
pi: 0.0026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGCGAACCAAAGGGAATGAT	ATTGGTGGAAAAGGGAGTCG	45.0	50.0	57.8	57.8	544 bp	1.0	76.8
rpl22_p2	TGCGAACCAAAGGGAATGAT	GTGGAAAAGGGAGTCGCTTT	45.0	50.0	57.8	58.4	540 bp	1.0	75.3
rpl22_p3	TGCGAACCAAAGGGAATGAT	GATCTCGTCGCCAATCTCAT	45.0	50.0	57.8	57.6	602 bp	1.0	75.0
rpl22_p4	GCGAACCAAAGGGAATGATG	ATTGGTGGAAAAGGGAGTCG	50.0	50.0	57.4	57.8	543 bp	1.0	74.0
rpl22_p5	TGCGAACCAAAGGGAATGAT	AGATCTCGTCGCCAATCTCA	45.0	50.0	57.8	58.6	603 bp	1.0	73.6