Hotspot

ycf2-trnI-CAU

IRa · 172781-173150 · 370 bp

Back to Zantedeschia

Pi 0.0415

MarkerSeek score 76.80

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 370
variable_sites: 12
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TGAACCGAATTTGATCCACGA	GTTGGGCGCTTTAACCATTC	42.9	50.0	58.0	58.0	429-510 bp	1.0	77.6
ycf2-trnI-CAU_p2	CTGAACCGAATTTGATCCACG	GTTGGGCGCTTTAACCATTC	47.6	50.0	57.6	58.0	430-511 bp	1.0	74.8
ycf2-trnI-CAU_p3	TGAACCGAATTTGATCCACGA	AGTTGGGCGCTTTAACCATT	42.9	45.0	58.0	58.4	430-511 bp	1.0	74.7
ycf2-trnI-CAU_p4	TGAACCGAATTTGATCCACGA	TTGGGCGCTTTAACCATTCA	42.9	45.0	58.0	58.4	428-509 bp	1.0	74.6
ycf2-trnI-CAU_p5	TGAACCGAATTTGATCCACG	GTTGGGCGCTTTAACCATTC	45.0	50.0	56.4	58.0	429-510 bp	1.0	73.5