Hotspot

rbcL-accD

LSC · 60023-63551 · 3529 bp

Back to Zantedeschia

Pi 0.00329

MarkerSeek score 59.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 3529
variable_sites: 10
pi: 0.00329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACCGCGCATGCTAGATAAAA	TGCCTGTGCCTTCTTTCATT	45.0	45.0	58.1	58.0	1826-2216 bp	1.0	84.0
rbcL-accD_p2	ACCGCGCATGCTAGATAAAA	TTGATGCCTGTGCCTTCTTT	45.0	45.0	58.1	58.0	1830-2220 bp	1.0	84.0
rbcL-accD_p3	ACCGCGCATGCTAGATAAAA	TTTGATGCCTGTGCCTTCTT	45.0	45.0	58.1	58.0	1831-2221 bp	1.0	84.0
rbcL-accD_p4	AAAAGCGAAGAGAGCATCGT	TGCCTGTGCCTTCTTTCATT	45.0	45.0	57.9	58.0	1591-1981 bp	1.0	83.8
rbcL-accD_p5	ACCGCGCATGCTAGATAAAA	ATGTGCTCCCTCCTTCTCAT	45.0	50.0	58.1	58.1	909-993 bp	1.0	83.5