Hotspot

trnG-GCC-trnfM-CAU

LSC · 38683-38844 · 162 bp

Back to Zantedeschia

Pi 0.00617

MarkerSeek score 56.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 1
pi: 0.00617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CTTGCCAAGGAAAAGATGCG	GCAAGATTGGTTGGATTGGC	50.0	50.0	58.0	58.0	1156-1274 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p2	TATGACTCACGCCCCTCTAG	GCAAGATTGGTTGGATTGGC	55.0	50.0	58.0	58.0	2486-2604 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p3	CTTGCCAAGGAAAAGATGCG	GCAACAAGGTCCAGTTGGTA	50.0	50.0	58.0	58.0	589-707 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p4	CTTGCCAAGGAAAAGATGCG	GTAATAGTGCACCCACACGT	50.0	50.0	58.0	57.9	717-835 bp	1.0	79.3
trnG-GCC-trnfM-CAU_p5	ATCCCGCCGATGCATTTTAT	GCAACAAGGTCCAGTTGGTA	45.0	50.0	58.1	58.0	1233-1351 bp	1.0	79.1