Hotspot

trnY-GUA-trnE-UUC

LSC · 33130-33355 · 226 bp

Back to Zantedeschia

Pi 0
MarkerSeek score 53.40
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
226
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 AGGAAAGCGGCGAATTTTTG TCTAGCGGTTCAGGACATCT 45.0 50.0 57.9 57.9 389-391 bp 1.0 78.7
trnY-GUA-trnE-UUC_p2 CAAAGGAAAGCGGCGAATTT TCTAGCGGTTCAGGACATCT 45.0 50.0 57.9 57.9 392-394 bp 1.0 78.7
trnY-GUA-trnE-UUC_p3 AGGAAAGCGGCGAATTTTTG GTCTAGCGGTTCAGGACATC 45.0 55.0 57.9 57.8 390-392 bp 1.0 78.2
trnY-GUA-trnE-UUC_p4 CAAAGGAAAGCGGCGAATTT GTCTAGCGGTTCAGGACATC 45.0 55.0 57.9 57.8 393-395 bp 1.0 78.2
trnY-GUA-trnE-UUC_p5 AGGAAAGCGGCGAATTTTTG TATCGTCTAGCGGTTCAGGA 45.0 50.0 57.9 57.7 394-396 bp 1.0 77.2