Hotspot

ycf1

Genome · 120369-123998 · 3630 bp

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Pi 0.478
MarkerSeek score 77.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
Genome
length_bp
3630
variable_sites
1539
pi
0.478
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGACTCTTTGGAAATGGCTTCA CGCAAAGGGCAGGAATATCT 40.9 50.0 57.9 58.0 359 bp 0.5 42.9
ycf1_p2 AGACTCTTTGGAAATGGCTTCA GCCATCTGTCCGGAAATTCT 40.9 50.0 57.9 58.0 1058 bp 0.5 42.8
ycf1_p3 AGACTCTTTGGAAATGGCTTCA CCGTTCGGGTCGATAATTGT 40.9 50.0 57.9 58.1 519 bp 0.5 42.6
ycf1_p4 AGACTCTTTGGAAATGGCTTCA CCTGATCTTTCTTGGGCGAA 40.9 50.0 57.9 57.9 493 bp 0.5 42.3
ycf1_p5 AGACTCTTTGGAAATGGCTTCA TCTCTGTATTCCGTTCGGGT 40.9 50.0 57.9 58.2 529 bp 0.5 41.9