Hotspot

ndhI

Genome · 128230-128787 · 558 bp

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Pi 0.464
MarkerSeek score 81.90
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
Genome
length_bp
558
variable_sites
236
pi
0.464
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 AGTGACGCTCTCATCGACTA AAAGTGATGCCATCTCCGAC 50.0 50.0 58.0 58.0 1892 bp 0.5 59.6
ndhI_p2 ATTCTCTATTTGGGCGGCTG AAAGTGATGCCATCTCCGAC 50.0 50.0 58.0 58.0 1605 bp 0.5 59.5
ndhI_p3 AGTGACGCTCTCATCGACTA TCTACCAGTTCTCCCCGATC 50.0 55.0 58.0 57.9 1284 bp 0.5 59.2
ndhI_p4 ATTCTCTATTTGGGCGGCTG TCTACCAGTTCTCCCCGATC 50.0 55.0 58.0 57.9 997 bp 0.5 59.2
ndhI_p5 AGTGACGCTCTCATCGACTA GTCCCAATGAAAGGGCAGAA 50.0 50.0 58.0 58.1 1711 bp 0.5 59.2