Hotspot

ndhG

Genome · 129120-129662 · 543 bp

Pi 0.495

MarkerSeek score 75.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 543
variable_sites: 191
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GATCGGGGAGAACTGGTAGA	GCTCAACTGCGTTAGTCAGA	55.0	50.0	57.9	57.9	1560 bp	0.5	58.9
ndhG_p2	TATGTGTTCGTGTATGCCCG	GCTCAACTGCGTTAGTCAGA	50.0	50.0	58.1	57.9	1919 bp	0.5	58.9
ndhG_p3	GATCAGATTGCTCTAGGGCG	GCTCAACTGCGTTAGTCAGA	55.0	50.0	57.9	57.9	1713 bp	0.5	58.9
ndhG_p4	TTGCCTGTCGATGACTGAAG	GCTCAACTGCGTTAGTCAGA	50.0	50.0	57.9	57.9	1774 bp	0.5	58.8
ndhG_p5	GATCGGGGAGAACTGGTAGA	TCTAACCATGTTCCGGCTTG	55.0	50.0	57.9	57.9	1105 bp	0.5	58.6