Pi
0.477
MarkerSeek score
79.40
Primer available
yes
Label
ndhA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhA_p1 | AGGACCTATGCTACGAGCTT | CGTTCCGATGGGATCAACTT | 50.0 | 50.0 | 57.9 | 58.0 | 2913 bp | 0.5 | 59.2 |
| ndhA_p2 | ATTGGGGCTTATCAGGACCT | CGTTCCGATGGGATCAACTT | 50.0 | 50.0 | 58.1 | 58.0 | 2926 bp | 0.5 | 59.1 |
| ndhA_p3 | AGGACCTATGCTACGAGCTT | ATTCGAAATGAATCCGCCCA | 50.0 | 45.0 | 57.9 | 57.9 | 2939 bp | 0.5 | 59.0 |
| ndhA_p4 | ATTGGGGCTTATCAGGACCT | ATTCGAAATGAATCCGCCCA | 50.0 | 45.0 | 58.1 | 57.9 | 2952 bp | 0.5 | 58.9 |
| ndhA_p5 | AGGACCTATGCTACGAGCTT | CGGGCATACACGAACACATA | 50.0 | 50.0 | 57.9 | 58.1 | 2983 bp | 0.5 | 58.8 |