Hotspot

chlN

Genome · 117249-118085 · 837 bp

Pi 0.488

MarkerSeek score 80.30

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 837
variable_sites: 340
pi: 0.488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	AGGTTTTGCTGCTCCATTGA	CGGGTTGTAACTCACGCATA	45.0	50.0	58.0	58.0	1705 bp	0.5	60.0
chlN_p2	TTCGATTAGCAGGCTTGGTC	CGGGTTGTAACTCACGCATA	50.0	50.0	58.0	58.0	1580 bp	0.5	59.8
chlN_p3	ATGGTTGAATCCGAGCCATC	CGGGTTGTAACTCACGCATA	50.0	50.0	58.0	58.0	1431 bp	0.5	59.8
chlN_p4	ACTTCGATTAGCAGGCTTGG	CGGGTTGTAACTCACGCATA	50.0	50.0	58.0	58.0	1582 bp	0.5	59.8
chlN_p5	CTTCGATTAGCAGGCTTGGT	CGGGTTGTAACTCACGCATA	50.0	50.0	58.0	58.0	1581 bp	0.5	59.8