Hotspot

psaC-ndhD

Genome · 130833-130970 · 138 bp

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Pi 0.408
MarkerSeek score 77.10
Primer available yes
Label psaC-ndhD

Feature Metadata

label_name
psaC-ndhD
feature_type
igs
region
Genome
length_bp
138
variable_sites
53
pi
0.408
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhD_p1 ACGCGTTTATTTGTGGCATG TGGGATTAATAAACCCGCGG 45.0 50.0 58.0 58.0 256 bp 0.5 59.6
psaC-ndhD_p2 ACGCGTTTATTTGTGGCATG TTGATGGCATGAGCGATGAA 45.0 45.0 58.0 58.0 861 bp 0.5 59.5
psaC-ndhD_p3 ACGCGTTTATTTGTGGCATG TTTGATGGCATGAGCGATGA 45.0 45.0 58.0 58.0 862 bp 0.5 59.5
psaC-ndhD_p4 GACTTCTTGAGTGTACGCGT TGGGATTAATAAACCCGCGG 50.0 50.0 58.0 58.0 270 bp 0.5 59.4
psaC-ndhD_p5 GACTTCTTGAGTGTACGCGT TTGATGGCATGAGCGATGAA 50.0 45.0 58.0 58.0 875 bp 0.5 59.3