Hotspot

ycf1-rps15

Genome · 123999-124428 · 430 bp

Back to Zamia

Pi 0.456
MarkerSeek score 79.30
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
Genome
length_bp
430
variable_sites
188
pi
0.456
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 GGCAGAATCCATAGCGAGAG TTCGGTGCAGTTCCAAATGA 55.0 45.0 57.9 58.0 1215 bp 0.5 59.2
ycf1-rps15_p2 GCCATTCCGGTTATCTGAGG TTCGGTGCAGTTCCAAATGA 55.0 45.0 58.1 58.0 1029 bp 0.5 58.9
ycf1-rps15_p3 AGTGGAATGAAGGATCCGGA TTCGGTGCAGTTCCAAATGA 50.0 45.0 58.1 58.0 1160 bp 0.5 58.8
ycf1-rps15_p4 GGCAGAATCCATAGCGAGAG TTTCTCTAGCAAGCACGGTC 55.0 50.0 57.9 57.9 1491 bp 0.5 58.7
ycf1-rps15_p5 TGCCCTATCTTCGAACAGGA TTCGGTGCAGTTCCAAATGA 50.0 45.0 58.1 58.0 1129 bp 0.5 58.7