Hotspot

chlN-ycf1

Genome · 118086-120368 · 2283 bp

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Pi 0.493
MarkerSeek score 79.60
Primer available yes
Label chlN-ycf1

Feature Metadata

label_name
chlN-ycf1
feature_type
igs
region
Genome
length_bp
2283
variable_sites
975
pi
0.493
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlN-ycf1_p1 CCGTCAAGGTATCAGGTTGG CGCAAAGGGCAGGAATATCT 55.0 50.0 58.0 58.0 2886 bp 0.5 59.6
chlN-ycf1_p2 CCGTCAAGGTATCAGGTTGG CCATCTCATGGGACTTGTGG 55.0 55.0 58.0 58.0 2741 bp 0.5 59.4
chlN-ycf1_p3 GGTTGGTTACCCGCTCATAG CGCAAAGGGCAGGAATATCT 55.0 50.0 58.1 58.0 2872 bp 0.5 59.4
chlN-ycf1_p4 GGTTGGTTACCCGCTCATAG CCATCTCATGGGACTTGTGG 55.0 55.0 58.1 58.0 2727 bp 0.5 59.2
chlN-ycf1_p5 TACTCAGGGGGAAGACACTG CCATCTCATGGGACTTGTGG 55.0 55.0 58.1 58.0 2965 bp 0.5 59.1