Hotspot

trnN-GTT-chlL

Genome · 115107-116331 · 1225 bp

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Pi 0.16
MarkerSeek score 80.50
Primer available yes
Label trnN-GTT-chlL

Feature Metadata

label_name
trnN-GTT-chlL
feature_type
igs
region
Genome
length_bp
1225
variable_sites
193
pi
0.16
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GTT-chlL_p1 TGAACCTACGACCAGTCAGT TCAATGGAGCAGCAAAACCT 50.0 45.0 58.0 58.0 1690 bp 0.5 59.9
trnN-GTT-chlL_p2 TTTCGTACAGTACCCCTGGT TCAATGGAGCAGCAAAACCT 50.0 45.0 58.0 58.0 2309 bp 0.5 59.9
trnN-GTT-chlL_p3 TGAACCTACGACCAGTCAGT GACCAAGCCTGCTAATCGAA 50.0 50.0 58.0 58.0 1815 bp 0.5 59.7
trnN-GTT-chlL_p4 TGAACCTACGACCAGTCAGT GATGGCTCGGATTCAACCAT 50.0 50.0 58.0 58.0 1964 bp 0.5 59.7
trnN-GTT-chlL_p5 TTTCGTACAGTACCCCTGGT GACCAAGCCTGCTAATCGAA 50.0 50.0 58.0 58.0 2434 bp 0.5 59.7