Hotspot

ycf2

IRb · 91049-98277 · 7229 bp

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Pi 0.00936

MarkerSeek score 51.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 7229
variable_sites: 143
pi: 0.00936
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GATGAGTCCCCTGTCTCTGT	TTCGAAGTGCTTTTCTTGCG	55.0	45.0	58.1	57.6	144-199 bp	1.0	87.8
ycf2_p2	GATGAGTCCCCTGTCTCTGT	AGTGCTTTTCTTGCGCCTTA	55.0	45.0	58.1	58.4	139-194 bp	1.0	87.7
ycf2_p3	AGATGAGTCCCCTGTCTCTG	TTCGAAGTGCTTTTCTTGCG	55.0	45.0	57.8	57.6	145-200 bp	1.0	87.7
ycf2_p4	AGATGAGTCCCCTGTCTCTG	AGTGCTTTTCTTGCGCCTTA	55.0	45.0	57.8	58.4	140-195 bp	1.0	87.6
ycf2_p5	AGTCCCCTGTCTCTGTGTTT	TTCGAAGTGCTTTTCTTGCG	50.0	45.0	58.2	57.6	140-195 bp	1.0	87.4