Hotspot

ycf1

SSC · 128179-133209 · 5031 bp

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Pi 0.0188

MarkerSeek score 54.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5031
variable_sites: 206
pi: 0.0188
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGCAGCACAAATTCTGGGA	TCAACGTTGGACAGCTGAAA	45.0	45.0	58.0	58.0	578-598 bp	1.0	90.5
ycf1_p2	AGCAGCACAAATTCTGGGAA	TCAACGTTGGACAGCTGAAA	45.0	45.0	58.0	58.0	577-597 bp	1.0	90.5
ycf1_p3	GTAGCTCAATGGCACGATCA	TCAACGTTGGACAGCTGAAA	50.0	45.0	58.1	58.0	815-835 bp	1.0	88.2
ycf1_p4	TGCTTAATTTGTTGCCCCCT	TCAACGTTGGACAGCTGAAA	45.0	45.0	58.0	58.0	1054-1074 bp	1.0	87.4
ycf1_p5	GCGTTCTTTGCTCCACAATC	TCAACGTTGGACAGCTGAAA	50.0	45.0	58.0	58.0	797-809 bp	0.5	63.6