Hotspot

accD

LSC · 60142-62987 · 2846 bp

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Pi 0.0459

MarkerSeek score 77.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2846
variable_sites: 243
pi: 0.0459
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTGGAGTTGCGATGAGAGTG	CTTAGTCCACACCTGTGCTC	50.0	55.0	57.9	57.9	642-982 bp	0.833333	84.0
accD_p2	TTGGAGTTGCGATGAGAGTG	AATGTGAACCCCCTGCTTTT	50.0	45.0	57.9	57.9	285-625 bp	0.833333	83.8
accD_p3	GTTCCGTTTGTGGTGAGAGT	CTTAGTCCACACCTGTGCTC	50.0	55.0	58.1	57.9	613-759 bp	0.666667	77.6
accD_p4	GTTCCGTTTGTGGTGAGAGT	AAATGTGAACCCCCTGCTTT	50.0	45.0	58.1	57.9	257-404 bp	0.666667	77.4
accD_p5	GTTCCGTTTGTGGTGAGAGT	AATGTGAACCCCCTGCTTTT	50.0	45.0	58.1	57.9	256-403 bp	0.666667	77.4