Hotspot

trnN-GUU-ndhF

SSC · 114090-115085 · 996 bp

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Pi 0.122

MarkerSeek score 77.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 996
variable_sites: 203
pi: 0.122
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CCCCGTAACTCTTCTTCAGC	TAGGAACTGGGAGTGGAAGG	55.0	55.0	58.0	58.0	988-1655 bp	0.833333	84.9
trnN-GUU-ndhF_p2	CCCGTAACTCTTCTTCAGCC	TAGGAACTGGGAGTGGAAGG	55.0	55.0	58.0	58.0	987-1654 bp	0.833333	84.9
trnN-GUU-ndhF_p3	GCCCCGTAACTCTTCTTCAG	TAGGAACTGGGAGTGGAAGG	55.0	55.0	58.0	58.0	989-1656 bp	0.833333	84.9
trnN-GUU-ndhF_p4	CCCCGTAACTCTTCTTCAGC	CAAGAGGTCGTGTGAACCAA	55.0	50.0	58.0	58.1	1441-2108 bp	0.833333	84.8
trnN-GUU-ndhF_p5	CCCGTAACTCTTCTTCAGCC	CAAGAGGTCGTGTGAACCAA	55.0	50.0	58.0	58.1	1440-2107 bp	0.833333	84.8