Hotspot

trnR-ACG-trnN-GUU

IRb · 113270-114017 · 748 bp

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Pi 0.066
MarkerSeek score 77.10
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
748
variable_sites
98
pi
0.066
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.74
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CCTCGCAATCACTAGCCAAT CACTACGAAGAAGTTCCGGG 50.0 55.0 58.0 58.0 1411-1485 bp 0.833333 82.5
trnR-ACG-trnN-GUU_p2 AAAAGCTCTGTCCTCCCTCT CACTACGAAGAAGTTCCGGG 50.0 55.0 58.0 58.0 1493-1567 bp 0.833333 82.2
trnR-ACG-trnN-GUU_p3 AAAAGCTCTGTCCTCCCTCT CCACTACGAAGAAGTTCCGG 50.0 55.0 58.0 58.0 1494-1568 bp 0.833333 82.2
trnR-ACG-trnN-GUU_p4 CCTTGTTCCTACATGACCCG CACTACGAAGAAGTTCCGGG 55.0 55.0 58.0 58.0 1578-1652 bp 0.833333 82.0
trnR-ACG-trnN-GUU_p5 CCTTGTTCCTACATGACCCG CCACTACGAAGAAGTTCCGG 55.0 55.0 58.0 58.0 1579-1653 bp 0.833333 82.0