Hotspot

ycf2-trnL-CAA

IRb · 98278-98978 · 701 bp

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Pi 0.066

MarkerSeek score 89.40

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 701
variable_sites: 91
pi: 0.066
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	CCCATTCCTTAGCCGGAAAG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.3	58.0	792-808 bp	1.0	89.4
ycf2-trnL-CAA_p2	CCCATTCCTTAGCCGGAAAG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.3	58.0	794-810 bp	1.0	89.4
ycf2-trnL-CAA_p3	GCCGGAAAGATATGTGACCA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.7	58.0	781-797 bp	1.0	89.3
ycf2-trnL-CAA_p4	GCCGGAAAGATATGTGACCA	TGAAATGGTAGACACGCGAG	50.0	50.0	57.7	58.0	783-799 bp	1.0	89.3
ycf2-trnL-CAA_p5	ATTTCCCATTCCTTAGCCGG	AAATGGTAGACACGCGAGAC	50.0	50.0	57.6	58.0	796-812 bp	1.0	89.0