Hotspot

trnI-CAU-ycf2

IRb · 90827-91048 · 222 bp

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Pi 0.0571

MarkerSeek score 82.20

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 222
variable_sites: 23
pi: 0.0571
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TGAATTGAATTGGGTCCATGA	50.0	38.1	58.0	55.4	357-361 bp	1.0	60.6
trnI-CAU-ycf2_p2	ATTGGCATGCATCCAGTAGG	TGAATTGAATTGGGTCCATGA	50.0	38.1	58.0	55.4	406-410 bp	1.0	59.9
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	TGAATTGGGTCCATGAATCT	50.0	40.0	58.0	54.3	352-356 bp	1.0	59.3
trnI-CAU-ycf2_p4	ATTGGCATGCATCCAGTAGG	TGAATTGGGTCCATGAATCT	50.0	40.0	58.0	54.3	401-405 bp	1.0	58.6
trnI-CAU-ycf2_p5	ATTGGCATGCATCCAGTAGG	TGAATTGAATTGGGTCCATGAA	50.0	36.4	58.0	56.2	406-410 bp	1.0	58.1