Hotspot

rpl14-rpl16

LSC · 85312-85526 · 215 bp

Back to Zabelia

Pi 0.0322

MarkerSeek score 82.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 17
pi: 0.0322
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	CGGGCCGCATACTTTATGAA	50.0	50.0	58.0	58.4	382-397 bp	1.0	82.7
rpl14-rpl16_p2	AGCATATCGGCGATTGCTAG	CGGGCCGCATACTTTATGAA	50.0	50.0	58.0	58.4	418-433 bp	1.0	82.4
rpl14-rpl16_p3	CATATCGGCGATTGCTAGCT	CGGGCCGCATACTTTATGAA	50.0	50.0	58.0	58.4	416-431 bp	1.0	82.4
rpl14-rpl16_p4	GAGCATATCGGCGATTGCTA	CGGGCCGCATACTTTATGAA	50.0	50.0	58.0	58.4	419-434 bp	1.0	82.3
rpl14-rpl16_p5	CCGCTGTTATCCGCTACATT	CGGGCCGCATACTTTATGAA	50.0	50.0	58.1	58.4	363-378 bp	1.0	82.1