Hotspot

psaA-ycf3

LSC · 44137-44938 · 802 bp

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Pi 0.0157

MarkerSeek score 72.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 30
pi: 0.0157
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCAGGTTTAGCCCATTCCTC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	57.9	58.0	985-1008 bp	1.0	84.0
psaA-ycf3_p2	CCAGGTTTAGCCCATTCCTC	GGAAACAGGCTATAGCGCTT	55.0	50.0	57.9	58.0	967-990 bp	1.0	84.0
psaA-ycf3_p3	CCAGGTTTAGCCCATTCCTC	TTGGTTGAAGATCACCAGGC	55.0	50.0	57.9	58.1	918-941 bp	1.0	83.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	958-981 bp	1.0	83.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	976-999 bp	1.0	83.5