primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	ACAATCTCAGCAAGTAGGCG	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027276	312	661	1240-1240	0.166667	46.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ACAATCTCAGCAAGTAGGCG	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027276	312	661	1246-1246	0.166667	46.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACAATCTCAGCAAGTAGGCG	GGAGCTTAGTTTCCGTCTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027276	312	661	1241-1241	0.166667	46.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCTCAGCAAGTAGGCG	GGGAGCTTAGTTTCCGTCTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027276	312	661	1242-1242	0.166667	46.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCTCAGCAAGTAGGCG	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	58.0	58.0	0.0	10.2	0.058422	312	661	1002-1002	0.166667	46.2
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	38428	38750	478-483	1.000000	82.8
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	38428	38750	476-481	1.000000	82.4
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	20	20	50.0	50.0	57.8	57.3	0.0	0.0	0.941166	38428	38750	451-456	1.000000	78.6
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	GCTTCTCCTGATGGTTGGTC	TACCATTCGACCATATCCGC	20	20	55.0	50.0	58.3	57.3	0.0	0.0	0.992148	38428	38750	449-454	1.000000	78.3
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	0.0	1.1	1.036941	38428	38750	472-477	1.000000	77.6
psaA-ycf3_p1	psaA-ycf3	1	CCAGGTTTAGCCCATTCCTC	ATCAAGCCGCTGAGTATTGG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.099551	44137	44938	985-1008	1.000000	84.0
psaA-ycf3_p2	psaA-ycf3	2	CCAGGTTTAGCCCATTCCTC	GGAAACAGGCTATAGCGCTT	20	20	55.0	50.0	57.9	58.0	0.0	18.7	0.103666	44137	44938	967-990	1.000000	84.0
psaA-ycf3_p3	psaA-ycf3	3	CCAGGTTTAGCCCATTCCTC	TTGGTTGAAGATCACCAGGC	20	20	55.0	50.0	57.9	58.1	0.0	3.2	0.148893	44137	44938	918-941	1.000000	83.9
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	44137	44938	958-981	1.000000	83.6
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	44137	44938	976-999	1.000000	83.5
accD_p1	accD	1	TTGGAGTTGCGATGAGAGTG	CTTAGTCCACACCTGTGCTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.170664	60570	60689	642-982	0.833333	84.0
accD_p2	accD	2	TTGGAGTTGCGATGAGAGTG	AATGTGAACCCCCTGCTTTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.186249	60570	60689	285-625	0.833333	83.8
accD_p3	accD	3	GTTCCGTTTGTGGTGAGAGT	CTTAGTCCACACCTGTGCTC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.136589	60570	60689	613-759	0.666667	77.6
accD_p4	accD	4	GTTCCGTTTGTGGTGAGAGT	AAATGTGAACCCCCTGCTTT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.152174	60570	60689	257-404	0.666667	77.4
accD_p5	accD	5	GTTCCGTTTGTGGTGAGAGT	AATGTGAACCCCCTGCTTTT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.152174	60570	60689	256-403	0.666667	77.4
rps12-clpP_p1	rps12-clpP	1	CTGAACGAGTCGCACATACA	ATGCAAGCTGTGAAACCAGA	20	20	50.0	45.0	58.0	58.0	13.6	0.0	0.037339	75282	75439	671-679	1.000000	88.8
rps12-clpP_p2	rps12-clpP	2	CTGAACGAGTCGCACATACA	TGCAAGCTGTGAAACCAGAT	20	20	50.0	45.0	58.0	58.0	13.6	0.0	0.037339	75282	75439	670-678	1.000000	88.8
rps12-clpP_p3	rps12-clpP	3	CTGAACGAGTCGCACATACA	TATAAACTCTCCCGGCGGAT	20	20	50.0	50.0	58.0	58.0	13.6	30.8	0.028182	75282	75439	723-731	1.000000	88.2
rps12-clpP_p4	rps12-clpP	4	TACACCGGAGCCTTTCCTTA	TGCAAGCTGTGAAACCAGAT	20	20	50.0	45.0	58.1	58.0	9.5	0.0	0.073150	75282	75439	1146-1160	0.333333	54.8
rps12-clpP_p5	rps12-clpP	5	TACACCGGAGCCTTTCCTTA	TATAAACTCTCCCGGCGGAT	20	20	50.0	50.0	58.1	58.0	9.5	30.8	0.063993	75282	75439	1199-1213	0.333333	54.7
rpl14-rpl16_p1	rpl14-rpl16	1	ACACATCAATTCTCGAGCCC	CGGGCCGCATACTTTATGAA	20	20	50.0	50.0	58.0	58.4	7.8	15.4	0.441164	85312	85526	382-397	1.000000	82.7
rpl14-rpl16_p2	rpl14-rpl16	2	AGCATATCGGCGATTGCTAG	CGGGCCGCATACTTTATGAA	20	20	50.0	50.0	58.0	58.4	35.6	15.4	0.413138	85312	85526	418-433	1.000000	82.4
rpl14-rpl16_p3	rpl14-rpl16	3	CATATCGGCGATTGCTAGCT	CGGGCCGCATACTTTATGAA	20	20	50.0	50.0	58.0	58.4	28.9	15.4	0.413138	85312	85526	416-431	1.000000	82.4
rpl14-rpl16_p4	rpl14-rpl16	4	GAGCATATCGGCGATTGCTA	CGGGCCGCATACTTTATGAA	20	20	50.0	50.0	58.0	58.4	36.0	15.4	0.415034	85312	85526	419-434	1.000000	82.3
rpl14-rpl16_p5	rpl14-rpl16	5	CCGCTGTTATCCGCTACATT	CGGGCCGCATACTTTATGAA	20	20	50.0	50.0	58.1	58.4	0.0	15.4	0.545461	85312	85526	363-378	1.000000	82.1
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TGAATTGAATTGGGTCCATGA	20	21	50.0	38.1	58.0	55.4	7.6	0.0	3.571201	90827	91048	357-361	1.000000	60.6
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	ATTGGCATGCATCCAGTAGG	TGAATTGAATTGGGTCCATGA	20	21	50.0	38.1	58.0	55.4	21.4	0.0	3.570880	90827	91048	406-410	1.000000	59.9
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	TGAATTGGGTCCATGAATCT	20	20	50.0	40.0	58.0	54.3	7.6	0.0	3.741702	90827	91048	352-356	1.000000	59.3
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	ATTGGCATGCATCCAGTAGG	TGAATTGGGTCCATGAATCT	20	20	50.0	40.0	58.0	54.3	21.4	0.0	3.741380	90827	91048	401-405	1.000000	58.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	ATTGGCATGCATCCAGTAGG	TGAATTGAATTGGGTCCATGAA	20	22	50.0	36.4	58.0	56.2	21.4	0.0	3.795372	90827	91048	406-410	1.000000	58.1
ycf2_p1	ycf2	1	GATGAGTCCCCTGTCTCTGT	TTCGAAGTGCTTTTCTTGCG	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.523996	97879	97998	144-199	1.000000	87.8
ycf2_p2	ycf2	2	GATGAGTCCCCTGTCTCTGT	AGTGCTTTTCTTGCGCCTTA	20	20	55.0	45.0	58.1	58.4	0.0	4.2	0.536094	97879	97998	139-194	1.000000	87.7
ycf2_p3	ycf2	3	AGATGAGTCCCCTGTCTCTG	TTCGAAGTGCTTTTCTTGCG	20	20	55.0	45.0	57.8	57.6	0.0	0.0	0.540938	97879	97998	145-200	1.000000	87.7
ycf2_p4	ycf2	4	AGATGAGTCCCCTGTCTCTG	AGTGCTTTTCTTGCGCCTTA	20	20	55.0	45.0	57.8	58.4	0.0	4.2	0.553036	97879	97998	140-195	1.000000	87.6
ycf2_p5	ycf2	5	AGTCCCCTGTCTCTGTGTTT	TTCGAAGTGCTTTTCTTGCG	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.575484	97879	97998	140-195	1.000000	87.4
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	CCCATTCCTTAGCCGGAAAG	AAATGGTAGACACGCGAGAC	20	20	55.0	50.0	58.3	58.0	5.0	15.9	0.327493	98278	98978	792-808	1.000000	89.4
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	CCCATTCCTTAGCCGGAAAG	TGAAATGGTAGACACGCGAG	20	20	55.0	50.0	58.3	58.0	5.0	15.9	0.328863	98278	98978	794-810	1.000000	89.4
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	GCCGGAAAGATATGTGACCA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.7	58.0	5.0	15.9	0.333413	98278	98978	781-797	1.000000	89.3
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	GCCGGAAAGATATGTGACCA	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	57.7	58.0	5.0	15.9	0.334784	98278	98978	783-799	1.000000	89.3
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	ATTTCCCATTCCTTAGCCGG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.6	58.0	0.0	15.9	0.372803	98278	98978	796-812	1.000000	89.0
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CCTCGCAATCACTAGCCAAT	CACTACGAAGAAGTTCCGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.048789	113270	114017	1411-1485	0.833333	82.5
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	AAAAGCTCTGTCCTCCCTCT	CACTACGAAGAAGTTCCGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.045216	113270	114017	1493-1567	0.833333	82.2
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	AAAAGCTCTGTCCTCCCTCT	CCACTACGAAGAAGTTCCGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.045216	113270	114017	1494-1568	0.833333	82.2
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CCTTGTTCCTACATGACCCG	CACTACGAAGAAGTTCCGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.032006	113270	114017	1578-1652	0.833333	82.0
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CCTTGTTCCTACATGACCCG	CCACTACGAAGAAGTTCCGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.032006	113270	114017	1579-1653	0.833333	82.0
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CCCCGTAACTCTTCTTCAGC	TAGGAACTGGGAGTGGAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.058919	114090	115085	988-1655	0.833333	84.9
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CCCGTAACTCTTCTTCAGCC	TAGGAACTGGGAGTGGAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.058919	114090	115085	987-1654	0.833333	84.9
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GCCCCGTAACTCTTCTTCAG	TAGGAACTGGGAGTGGAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.058919	114090	115085	989-1656	0.833333	84.9
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CCCCGTAACTCTTCTTCAGC	CAAGAGGTCGTGTGAACCAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.063436	114090	115085	1441-2108	0.833333	84.8
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CCCGTAACTCTTCTTCAGCC	CAAGAGGTCGTGTGAACCAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.063436	114090	115085	1440-2107	0.833333	84.8
ycf1_p1	ycf1	1	AAGCAGCACAAATTCTGGGA	TCAACGTTGGACAGCTGAAA	20	20	45.0	45.0	58.0	58.0	0.0	27.0	0.031610	129234	129353	578-598	1.000000	90.5
ycf1_p2	ycf1	2	AGCAGCACAAATTCTGGGAA	TCAACGTTGGACAGCTGAAA	20	20	45.0	45.0	58.0	58.0	0.0	27.0	0.031610	129234	129353	577-597	1.000000	90.5
ycf1_p3	ycf1	3	GTAGCTCAATGGCACGATCA	TCAACGTTGGACAGCTGAAA	20	20	50.0	45.0	58.1	58.0	0.0	27.0	0.091284	129234	129353	815-835	1.000000	88.2
ycf1_p4	ycf1	4	TGCTTAATTTGTTGCCCCCT	TCAACGTTGGACAGCTGAAA	20	20	45.0	45.0	58.0	58.0	0.0	27.0	0.053976	129234	129353	1054-1074	1.000000	87.4
ycf1_p5	ycf1	5	GCGTTCTTTGCTCCACAATC	TCAACGTTGGACAGCTGAAA	20	20	50.0	45.0	58.0	58.0	0.0	27.0	0.062155	129234	129353	797-809	0.500000	63.6
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CACTACGAAGAAGTTCCGGG	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.032006	133751	134498	1578-1652	1.000000	91.7
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCACTACGAAGAAGTTCCGG	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.032006	133751	134498	1579-1653	1.000000	91.7
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CACTACGAAGAAGTTCCGGG	AAAAGCTCTGTCCTCCCTCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.045216	133751	134498	1493-1567	1.000000	91.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CCACTACGAAGAAGTTCCGG	AAAAGCTCTGTCCTCCCTCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.045216	133751	134498	1494-1568	1.000000	91.6
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CACTACGAAGAAGTTCCGGG	CCTCGCAATCACTAGCCAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.048789	133751	134498	1411-1485	1.000000	91.6
rrn16-trnV-GAC_p1	rrn16-trnV-GAC	1	CCGACTTGCATGTGTTAAGC	GGCAGGGAGGGGATATAACT	20	20	50.0	55.0	57.8	58.0	10.4	0.0	0.293591	142085	142342	394-414	1.000000	86.4
rrn16-trnV-GAC_p2	rrn16-trnV-GAC	2	CCGACTTGCATGTGTTAAGC	CGTGGTGGAAGTCATCAGTT	20	20	50.0	50.0	57.8	57.8	10.4	0.0	0.417143	142085	142342	352-372	1.000000	86.0
rrn16-trnV-GAC_p3	rrn16-trnV-GAC	3	CCGACTTGCATGTGTTAAGC	TTGACGTGGTGGAAGTCATC	20	20	50.0	50.0	57.8	57.8	10.4	0.0	0.411129	142085	142342	356-376	1.000000	85.9
rrn16-trnV-GAC_p4	rrn16-trnV-GAC	4	GCAGGTTCTTACGCGTTACT	GGCAGGGAGGGGATATAACT	20	20	50.0	55.0	58.3	58.0	20.8	0.0	0.339837	142085	142342	447-467	1.000000	85.5
rrn16-trnV-GAC_p5	rrn16-trnV-GAC	5	TTCTTACGCGTTACTCACCC	GGCAGGGAGGGGATATAACT	20	20	50.0	55.0	57.6	58.0	20.8	0.0	0.398783	142085	142342	442-462	1.000000	85.1
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	CCCATTCCTTAGCCGGAAAG	20	20	50.0	55.0	58.0	58.3	15.9	5.0	0.327493	148790	149490	792-808	1.000000	89.4
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	CCCATTCCTTAGCCGGAAAG	20	20	50.0	55.0	58.0	58.3	15.9	5.0	0.328863	148790	149490	794-810	1.000000	89.4
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AAATGGTAGACACGCGAGAC	GCCGGAAAGATATGTGACCA	20	20	50.0	50.0	58.0	57.7	15.9	5.0	0.333413	148790	149490	660-797	1.000000	89.3
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	GCCGGAAAGATATGTGACCA	20	20	50.0	50.0	58.0	57.7	15.9	5.0	0.334784	148790	149490	662-799	1.000000	89.3
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	ATTTCCCATTCCTTAGCCGG	20	20	50.0	50.0	58.0	57.6	15.9	0.0	0.372803	148790	149490	796-812	1.000000	89.0
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TGAATTGAATTGGGTCCATGA	GTTGGGCGCTTTAACCATTC	21	20	38.1	50.0	55.4	58.0	0.0	7.6	3.571201	156720	156941	357-361	1.000000	60.3
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TGAATTGAATTGGGTCCATGA	ATTGGCATGCATCCAGTAGG	21	20	38.1	50.0	55.4	58.0	0.0	21.4	3.570880	156720	156941	406-410	1.000000	59.7
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	TGAATTGGGTCCATGAATCT	GTTGGGCGCTTTAACCATTC	20	20	40.0	50.0	54.3	58.0	0.0	7.6	3.741702	156720	156941	352-356	1.000000	59.0
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TGAATTGGGTCCATGAATCT	ATTGGCATGCATCCAGTAGG	20	20	40.0	50.0	54.3	58.0	0.0	21.4	3.741380	156720	156941	401-405	1.000000	58.4
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	TGAATTGAATTGGGTCCATGAA	ATTGGCATGCATCCAGTAGG	22	20	36.4	50.0	56.2	58.0	0.0	21.4	3.795372	156720	156941	406-410	1.000000	57.9