Hotspot

ndhA

SSC · 114688-116775 · 2088 bp

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Pi 0.000931

MarkerSeek score 45.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2088
variable_sites: 8
pi: 0.000931
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2225-2251 bp	1.0	70.6
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2223-2249 bp	1.0	70.1
ndhA_p3	TGAACCCAGTTACCATAGGGA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	57.8	57.9	2228-2254 bp	1.0	69.6
ndhA_p4	TGAACCCAGTTACCATAGGGA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	57.8	57.8	2226-2252 bp	1.0	69.1
ndhA_p5	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2224-2250 bp	1.0	65.0