Hotspot

ndhF-rpl32

SSC · 107292-108201 · 910 bp

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Pi 0.00171
MarkerSeek score 52.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
910
variable_sites
7
pi
0.00171
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 1000-1021 bp 1.0 43.5
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 972-993 bp 1.0 43.3
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 973-994 bp 1.0 43.2
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 974-995 bp 1.0 43.2
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 975-996 bp 1.0 43.2