Hotspot

trnG-UCC-trnT-GGU

LSC · 14306-16277 · 1972 bp

Back to Yushania

Pi 0.00239

MarkerSeek score 40.60

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1972
variable_sites: 14
pi: 0.00239
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	CATCGTTAGCTTGGAAGGCT	GCGTTCAGACCAAAAATGCA	50.0	45.0	58.0	57.9	2941-2947 bp	1.0	79.2
trnG-UCC-trnT-GGU_p2	CATCGTTAGCTTGGAAGGCT	TGCTTCGTTTAGCGTTCAGA	50.0	45.0	58.0	57.9	2952-2958 bp	1.0	79.1
trnG-UCC-trnT-GGU_p3	TTTCCACCGAGCTGAAACAA	GCGTTCAGACCAAAAATGCA	45.0	45.0	58.0	57.9	2660-2666 bp	1.0	79.1
trnG-UCC-trnT-GGU_p4	TTTCCACCGAGCTGAAACAA	TGCTTCGTTTAGCGTTCAGA	45.0	45.0	58.0	57.9	2671-2677 bp	1.0	79.0
trnG-UCC-trnT-GGU_p5	CGTTAGCTTGGAAGGCTAGG	GCGTTCAGACCAAAAATGCA	55.0	45.0	58.1	57.9	2938-2944 bp	1.0	79.0