Hotspot

rps16-trnQ-UUG

LSC · 5566-7106 · 1541 bp

Back to Yushania

Pi 0.000725
MarkerSeek score 51.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1541
variable_sites
5
pi
0.000725
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1607-1615 bp 1.0 78.9
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1618-1626 bp 1.0 78.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1611-1619 bp 1.0 77.8
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1622-1630 bp 1.0 77.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAAACCAGAAATGGGGCGT 45.0 45.0 58.1 57.7 1667-1675 bp 1.0 77.1